Book Description:
Benson’s Microbiological Applications has been the “gold standard” of microbiology laboratory manuals for over 35 years. This manual has a number of attractive features that resulted in its adoption in universities, colleges, and community colleges. These features include user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. In revising the lab manual for the fourteenth edition, we have tried to maintain the proven strengths of the manual and further enhance it. We have updated the introductory material of the fungi, protozoa, and algae to reflect changes in scientific information. Finally, the names of microorganisms used by the American Type Culture Collection. This is important for those users who rely on the ATCC for a source of cultures.
Table of contents :
Preface……Page 2
Laboratory Protocol……Page 3
PART 1 Microscopy……Page 6
1 Brightfield Microscopy……Page 7
2 Darkfield Microscopy……Page 13
3 Phase-Contrast Microscopy……Page 15
4 Fluorescence Microscopy……Page 21
5 Microscopic Measurements……Page 26
PART 2 Survey of Microrganisms……Page 29
6 Protozoa, Algae, and Cyanobacteria……Page 30
7 Microscopic Invertebrates……Page 39
8 Aseptic Technique……Page 42
9 The Bacteria……Page 49
10 The Fungi: Yeasts and Molds……Page 51
PART 3 Microscope Slide Techniques (Bacterial Morphology)……Page 57
11 Negative Staining……Page 58
12 Smear Preparation……Page 60
13 Simple Staining……Page 64
14 Capsular Staining……Page 65
15 Gram Staining……Page 66
16 Spore Staining: Two Methods……Page 69
17 Acid-Fast Staining: Ziehl-Neelsen Method……Page 71
18 Acid-Fast Staining: Fluorescence Method……Page 72
19 Motility Determination……Page 74
PART 4 Culture Methods……Page 77
20 Culture Media Preparation……Page 78
21 Pure Culture Techniques……Page 84
22 Cultivation of Anaerobes……Page 91
23 Bacterial Population Counts……Page 95
24 Slide Culture: Autotrophs……Page 101
25 Slime Mold Culture……Page 102
26 Slide Culture: Molds……Page 105
27 Isolation of Anaerobic Phototrophic Bacteria: Using the Winogradsky Column……Page 108
PART 5 Bacterial Viruses: Isolation and Propagation……Page 112
28 Isolation of Phage from Sewage……Page 114
29 Isolation of Phage from Flies……Page 117
30 Burst Size Determination: A One-Step Growth Curve……Page 121
PART 6 Microbila Interrelationships……Page 126
31 Bacterial Commensalism……Page 127
32 Bacterial Synergism……Page 128
33 Microbial Antagonism……Page 129
PART 7 Environmental Influences and Control of Microbial Growth……Page 130
34 Temperature: Effects on Growth……Page 131
35 Temperature: Lethal Effects……Page 133
36 pH and Microbial Growth……Page 135
37 Osmotic Pressure and Bacterial Growth……Page 136
38 Oligodynamic Action……Page 137
39 Ultraviolet Light: Lethal Effects……Page 138
40 Evaluation of Disinfectants: The Use-Dilution Method……Page 140
41 Evaluation of Alcohol: Its Effectiveness as a Skin Degerming Agent……Page 142
42 Evaluation of Antiseptics: The Filter Paper Disk Method……Page 144
43 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method……Page 146
44 Effectiveness of Hand Scrubbing……Page 149
PART 8 Identification of Unknown Bacteria……Page 152
45 Preparation and Care of Stock Cultures……Page 153
46 Morphological Study of Unknown……Page 155
47 Cultural Characteristics……Page 158
48 Physiological Characteristics: Oxidation and Fermentation Tests……Page 162
49 Physiological Characteristics: Hydrolytic Reactions……Page 171
50 Physiological Characteristics: Miscellaneous Tests……Page 175
51 Use of Bergey’s Manual and Identibacter Interactus……Page 178
PART 9 Miniaturized Multitest Systems……Page 183
52 Enterobacteriaceae Identification: The API 20E System……Page 184
53 Enterobacteriaceae Identification: The Enterotube II System……Page 188
54 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System……Page 193
55 Staphylococcus Identification: The API Staph-Ident System……Page 197
PART 10 Microbiology of Soil……Page 200
56 Microbial Population Counts of Soil……Page 201
57 Isolation of an Antibiotic Producer from Soil……Page 202
58 The Nitrogen Cycle……Page 205
59 Nitrogen-Fixing Bacteria……Page 207
60 Ammonification in Soil……Page 211
61 Isolation of a Denitrifier from Soil: Using Nitrate Agar……Page 212
62 Isolation of a Denitrifier from Soil: Using an Enrichment Medium……Page 215
PART 11 Microbiology of Water……Page 219
63 Bacteriological Examination of Water: Qualitative Tests……Page 220
64 The Membrane Filter Method……Page 224
65 Standard Plate Count: A Quantitative Test……Page 226
PART 12 Microbiology of Milk and Food Products……Page 227
66 Standard Plate Count of Milk……Page 228
67 Direct Microscopic Count of Organisms in Milk: The Breed Count……Page 229
68 Reductase Test……Page 232
69 Bacterial Counts of Foods……Page 234
70 Microbial Spoilage of Canned Food……Page 235
71 Microbial Spoilage of Refrigerated Meat……Page 237
72 Microbiology of Alchohol Fermentation……Page 239
73 Microbiology of Yogurt Production……Page 241
PART 13 Bacterial Genetic Variations……Page 243
74 Mutant Isolation by Gradient Plate Method……Page 244
75 Mutant Isolation by Replica Plating……Page 246
76 Bacterial Mutagenicity and Carcinogenesis: The Ames Test……Page 248
PART 14 Medical Microbiology and Immunology……Page 251
77 A Synthetic Epidemic……Page 252
78 The Staphylococci: Isolation and Identification……Page 255
79 The Streptococci: Isolation and Identification……Page 260
80 Gram-Negative Intestinal Pathogens……Page 268
81 Urinary Tract Pathogens……Page 272
82 Slide Agglutination Test: Serological Typing……Page 277
83 Slide Agglutination (Latex) Test: For S. aureus Identification……Page 279
84 Tube Agglutination Test: The Heterophile Antibody Test……Page 281
85 Tube Agglutination Test: The Widal Test……Page 283
86 Phage Typing……Page 285
87 White Blood Cell Study: The Differential WBC Count……Page 286
88 Total WBC Count……Page 290
89 Blood Grouping……Page 294
90 The Snyder Caries Susceptibility Test……Page 297
LooseLeaf Benson’s Microbiological Applications Laboratory Manual- Concise Version
Author(s): Alfred E Brown Ph.D., Heidi Smith
Publisher: McGraw-Hill Education, Year: 2016
ISBN: 1259705234